Comparative physiological, ultrastructural and proteomic analyses reveal sexual differences in the responses of Populus cathayana under drought stress.
Identifieur interne : 003330 ( Main/Exploration ); précédent : 003329; suivant : 003331Comparative physiological, ultrastructural and proteomic analyses reveal sexual differences in the responses of Populus cathayana under drought stress.
Auteurs : Sheng Zhang [République populaire de Chine] ; Fugui Chen ; Shuming Peng ; Wujun Ma ; Helena Korpelainen ; Chunyang LiSource :
- Proteomics [ 1615-9861 ] ; 2010.
Descripteurs français
- KwdFr :
- Analyse de regroupements (MeSH), Caractères sexuels (MeSH), Cellules du mésophylle (cytologie), Cellules du mésophylle (ultrastructure), Complexe protéique du photosystème II (métabolisme), Photosynthèse (génétique), Populus (croissance et développement), Populus (métabolisme), Populus (physiologie), Populus (ultrastructure), Protéines végétales (génétique), Protéines végétales (métabolisme), Protéomique (méthodes), Réaction de polymérisation en chaîne (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Spectrométrie de masse (MeSH), Stress physiologique (MeSH), Sécheresses (MeSH), Électrophorèse bidimensionnelle sur gel (MeSH).
- MESH :
- croissance et développement : Populus.
- cytologie : Cellules du mésophylle.
- génétique : Photosynthèse, Protéines végétales.
- métabolisme : Complexe protéique du photosystème II, Populus, Protéines végétales.
- méthodes : Protéomique.
- physiologie : Populus.
- ultrastructure : Analyse de regroupements, Caractères sexuels, Cellules du mésophylle, Populus, Réaction de polymérisation en chaîne, Régulation de l'expression des gènes végétaux, Spectrométrie de masse, Stress physiologique, Sécheresses, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- Cluster Analysis (MeSH), Droughts (MeSH), Electrophoresis, Gel, Two-Dimensional (MeSH), Gene Expression Regulation, Plant (MeSH), Mass Spectrometry (MeSH), Mesophyll Cells (cytology), Mesophyll Cells (ultrastructure), Photosynthesis (genetics), Photosystem II Protein Complex (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Polymerase Chain Reaction (MeSH), Populus (growth & development), Populus (metabolism), Populus (physiology), Populus (ultrastructure), Proteomics (methods), Sex Characteristics (MeSH), Stress, Physiological (MeSH).
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Photosystem II Protein Complex, Plant Proteins.
- cytology : Mesophyll Cells.
- genetics : Photosynthesis.
- growth & development : Populus.
- metabolism : Populus.
- methods : Proteomics.
- physiology : Populus.
- ultrastructure : Mesophyll Cells, Populus.
- Cluster Analysis, Droughts, Electrophoresis, Gel, Two-Dimensional, Gene Expression Regulation, Plant, Mass Spectrometry, Polymerase Chain Reaction, Sex Characteristics, Stress, Physiological.
Abstract
Drought is a major abiotic stress, limiting the survival and growth of young plants. However, little is known about sex-dependent responses to drought at the proteome level. In this study, we carried out investigations on comparative proteomics, combined with physiological and organelle structure analyses, in males and females of Populus cathayana Rehd. Three-month-old poplar cuttings were treated at 30% of field capacity and at 100% field capacity as a control in a greenhouse for 40 days. Drought greatly inhibited plant growth, damaged the photosynthetic system and destructed the structures of chloroplasts, mitochondria and cellular membranes. However, males suffered less from the adverse effects of drought than did females. Using 2-DE, 563 spots were detected, of which 64 spots displayed significant drought effect and 44 spots displayed a significant sex by drought interaction effect. The results suggest that the different responses to drought stress detected between the sexes have a close relationship to the changes in the expression of sex-dependent proteins, including, e.g. photosynthesis-related proteins, homeostasis-related proteins and stress response proteins. These proteins could contribute to a physiological advantage under drought, giving potential clues for understanding sexual differences in the performance of plants in different environments.
DOI: 10.1002/pmic.200900650
PubMed: 20455211
Affiliations:
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type="abstract" xml:lang="en">Drought is a major abiotic stress, limiting the survival and growth of young plants. However, little is known about sex-dependent responses to drought at the proteome level. In this study, we carried out investigations on comparative proteomics, combined with physiological and organelle structure analyses, in males and females of Populus cathayana Rehd. Three-month-old poplar cuttings were treated at 30% of field capacity and at 100% field capacity as a control in a greenhouse for 40 days. Drought greatly inhibited plant growth, damaged the photosynthetic system and destructed the structures of chloroplasts, mitochondria and cellular membranes. However, males suffered less from the adverse effects of drought than did females. Using 2-DE, 563 spots were detected, of which 64 spots displayed significant drought effect and 44 spots displayed a significant sex by drought interaction effect. The results suggest that the different responses to drought stress detected between the sexes have a close relationship to the changes in the expression of sex-dependent proteins, including, e.g. photosynthesis-related proteins, homeostasis-related proteins and stress response proteins. These proteins could contribute to a physiological advantage under drought, giving potential clues for understanding sexual differences in the performance of plants in different environments.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20455211</PMID><DateCompleted><Year>2010</Year><Month>12</Month><Day>08</Day></DateCompleted><DateRevised><Year>2010</Year><Month>09</Month><Day>06</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1615-9861</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>14</Issue><PubDate><Year>2010</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Proteomics</Title><ISOAbbreviation>Proteomics</ISOAbbreviation></Journal><ArticleTitle>Comparative physiological, ultrastructural and proteomic analyses reveal sexual differences in the responses of Populus cathayana under drought stress.</ArticleTitle><Pagination><MedlinePgn>2661-77</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/pmic.200900650</ELocationID><Abstract><AbstractText>Drought is a major abiotic stress, limiting the survival and growth of young plants. However, little is known about sex-dependent responses to drought at the proteome level. In this study, we carried out investigations on comparative proteomics, combined with physiological and organelle structure analyses, in males and females of Populus cathayana Rehd. Three-month-old poplar cuttings were treated at 30% of field capacity and at 100% field capacity as a control in a greenhouse for 40 days. Drought greatly inhibited plant growth, damaged the photosynthetic system and destructed the structures of chloroplasts, mitochondria and cellular membranes. However, males suffered less from the adverse effects of drought than did females. Using 2-DE, 563 spots were detected, of which 64 spots displayed significant drought effect and 44 spots displayed a significant sex by drought interaction effect. The results suggest that the different responses to drought stress detected between the sexes have a close relationship to the changes in the expression of sex-dependent proteins, including, e.g. photosynthesis-related proteins, homeostasis-related proteins and stress response proteins. These proteins could contribute to a physiological advantage under drought, giving potential clues for understanding sexual differences in the performance of plants in different environments.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Sheng</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, P R China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Fugui</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Peng</LastName><ForeName>Shuming</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Wujun</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Korpelainen</LastName><ForeName>Helena</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Chunyang</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Proteomics</MedlineTA><NlmUniqueID>101092707</NlmUniqueID><ISSNLinking>1615-9853</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045332">Photosystem II Protein Complex</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016000" MajorTopicYN="N">Cluster Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055864" MajorTopicYN="Y">Droughts</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015180" MajorTopicYN="N">Electrophoresis, Gel, Two-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D058503" MajorTopicYN="N">Mesophyll Cells</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010788" MajorTopicYN="N">Photosynthesis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045332" MajorTopicYN="N">Photosystem II Protein Complex</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012727" MajorTopicYN="N">Sex Characteristics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="Y">Stress, Physiological</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>12</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20455211</ArticleId><ArticleId IdType="doi">10.1002/pmic.200900650</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chen, Fugui" sort="Chen, Fugui" uniqKey="Chen F" first="Fugui" last="Chen">Fugui Chen</name><name sortKey="Korpelainen, Helena" sort="Korpelainen, Helena" uniqKey="Korpelainen H" first="Helena" last="Korpelainen">Helena Korpelainen</name><name sortKey="Li, Chunyang" sort="Li, Chunyang" uniqKey="Li C" first="Chunyang" last="Li">Chunyang Li</name><name sortKey="Ma, Wujun" sort="Ma, Wujun" uniqKey="Ma W" first="Wujun" last="Ma">Wujun Ma</name><name sortKey="Peng, Shuming" sort="Peng, Shuming" uniqKey="Peng S" first="Shuming" last="Peng">Shuming Peng</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Sheng" sort="Zhang, Sheng" uniqKey="Zhang S" first="Sheng" last="Zhang">Sheng Zhang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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